Phospho-slp-76 ser376
WebThe mouse phospho-SLP-76 (Ser376) kit enables the cell-based quantitative detection of mouse phosphorylated SLP-76 at Ser376, for monitoring T-lymphocyte activation. See … Web15 min; green), using Phospho-SLP-76 (Ser376) (E3G9U) XP® Rabbit mAb #76384 (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Western blot analysis of extracts from Jurkat cells ...
Phospho-slp-76 ser376
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WebPE anti-SLP76 Phospho (Ser376) Download MSDS Product data sheet Product Type: Antibodies Single Brand: Sony Clone: A18001A Application: Intracellular Flow Cytometry Isotype: Mouse IgG1, κ Reactivity: … WebListed are ELISA Kits for the detection of SLP76, an alias name of lymphocyte cytosolic protein 2. The human protein, encoded by the gene LCP2, is 533 amino acid residues long and has a mass of 60,188 daltons. This protein is reported to have a cytoplasmic cellular localization. This target may also be known as: 76 kDa tyrosine phosphoprotein ...
WebAs Phospho SLP76 (Ser396) is a readout of T cell activation, it was used to monitor the inhibitory effect of PD-L1 recombinant protein on T cell activation. This assay was performed using the two-plate assay protocol. 400 000 Jurkat cells were plated in 25 µL of complete RPMI culture medium with 10% FBS. WebThe Mouse Total SLP-76 cellular assay kit is designed to monitor the expression level of SLP-76, whether phosphorylated or unphosphorylated. It is compatible with our Mouse phospho-SLP-76 (Ser376) kit, and enables the analysis of phosphorylated and total proteins from a single sample for better readouts of T-lymphocyte activation. Benefits
WebSLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus … Web76 kDa (SLP-76) is a hematopoietic adapter protein that is important in multiple biochemical signaling pathways and necessary for T cell development and activation (1). ZAP-70 …
WebJan 20, 2024 · We report here that SLP76-S376A-expressing mice exhibit normal thymocyte development and no detectable phenotypic alterations in mature T cell subsets or other lymphoid and myeloid cell lineages. Biochemical analyses revealed that mutant T cells were hypersensitive to TCR stimulation.
Webhuman phospho-SLP-76 (Ser376) as compared to kit #30794. Background: SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) is a hematopoietic adapter protein that is … the pet shop whitehall mallWebLymphocyte cytosolic protein (SLP-76) is required for T-cell activation and has been implicated in a number of signaling pathways. SLP76 is phosphorylated at Ser376 by HPK1, and this phosphorylation leads to a decrease in T-cell receptor signaling. o Description of the LANCE Ultra Assay the pet show brisbaneWebSLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. the pet show itvWebFeb 27, 2024 · Image: ELISA detection of phospho-SLP-76 (Ser376) using a FastScan™ sandwich ELISA kit from Cell Signaling Technology. Treatment efficacy is supported by Western blot analysis. Consider using automation the pet showerWebSLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T … thepetshow.comWebThis SLP-76 (Phospho-Ser376) TR-FRET Assay Kit uses a homogeneous TR-FRET assay method amenable to rapid measurement of phosphorylated protein levels in cells. This SLP-76 (Phospho-Ser376) TR-FRET Assay Kit is robust, with a Z’ value of 0.92, and suitable for screening a large number of samples. the pet show sydney promo codeWebSLP76 is a cytosolic adapter protein that functions as a substrate in the T cell receptor (TCR) activation pathway. Upon TCR engagement, SLP76 is phosphorylated on multiple residues, resulting in the protein being … the pet show sydney