Phm6 vector

http://www.protocol-online.org/biology-forums/posts/5295.html WebbpET SUMO (linearized) Linearized bacterial vector with 3'-T overhangs for TA cloning and inducible expression of an N-terminally 6xHis- and SUMO-tagged protein. Sequence …

Immunoprecipitation assay of lysates from Neuro-2a cells …

WebbVector Type Resistance Marker Bacterial Resistance Source Sequence Available; pHM6: Mammalian Expression: Neomycin: Ampicillin: Roche WebbThe pES6, pHM4, pHM5 and pHM6 vectors were derived from the pES4 plasmid containing bgl1, celA-celB, celA and celB synthetic genes, respectively. All of the genes were flanked by a synthetic... how do the seasons happen https://serranosespecial.com

pHM6 vector map and sequence - novoprolabs.com

WebbImmunoprecipitation assay of lysates from Neuro-2a cells transfected with pHM6 vector containing HA-tagged p190RhoGEF-C protein or from Neuro-2a cell transfected with … Webb11 juni 2024 · Read "10.1016/S1357-2725(01)00006-1" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. how much should i weigh if im 11 years old

pET SUMO (linearized) Sequence and Map - SnapGene

Category:10.1016/S1357-2725(01)00006-1 DeepDyve

Tags:Phm6 vector

Phm6 vector

Addgene: pHM6-alphasynuclein-WT

Webb1 aug. 2008 · The HA-tagged protein was obtained from a pHM6 vector encoding no protein (Mock). HA-tagged Identification of the domains responsible for interactions between Freud-1/Aki1 and PDK1. (A) The... Webb16 sep. 2009 · The expression plasmid containing the M gene (pHM6-m) or the NP gene (pHM6-np) was then constructed by inserting the M or NP gene into the pHM6 eukaryote …

Phm6 vector

Did you know?

WebbThe product is provided 'AS IS' and the viability of ATCC ® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the … Webb(a) Immunoblot analysis of MCF7 cells, which were stably transfected with HA-Aurora-A and pHM6 vector, with anti-HA and anti-ERα and anti-actin antibodies. (b–d) Aurora-A- and pHM6...

WebbPlasmid pHM6-alphasynuclein-WT from Dr. David Rubinsztein's lab contains the insert SNCA and is published in Biochem J. 2000 Mar 15;346 Pt 3:577-81. This plasmid is … Webb15 apr. 2010 · The PCR amplified merB::flag fragment (0.8 kb) was subcloned into the binary expression vector pHM6, downstream of the E12 promoter, the Cauliflower mosaic virus 35 S promoter with two enhancers in tandem, and an omega sequence, to create the plasmid pBMK01.

WebbOpen reading frame of c-myc was cloned into pHM6 vector. These PKM2, PKM2 mutants, and PKM1 expression vector carry mutations at the PKM2 siRNA targeting site, therefore there are resistant to siRNA knockdown. For bicistronic vectors, β-actin ORF was synthesized and inserted into pEGFP-N1 vector with HA tag in N-terminal of . WebbExpression Plasmid Vector Phm6, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, …

WebbPlasmid pHM6-alphasynuclein-A53T from Dr. David Rubinsztein's lab contains the insert SNCA and is published in Biochem J. 2000 Mar 15;346 Pt 3:577-81. This plasmid is …

Webb21 aug. 2008 · Identification of TUSC4/NPRL2 as a PDK1-interacting protein. (a) After cloning the full-length cDNA of each PDK1 binding protein (BP), we transfected a pc5FLAG vector encoding none (mock, lanes 1 and 2), full-length candidate proteins (BP1-BP8 and TUSC4, lanes 3–11), or 14-3-3θ (lane 12), together with a pHM6 vector encoding none (–, … how much should i weigh if im 10 years oldWebba pc5FLAG vector was generated by polymerase chain reaction (PCR) with an I.M.A.G.E clone (clone ID 6169151; Open Biosystems, Huntsville, AL, USA) as the template. The p60 c-src cDNA in a pHM6 vector was generated by PCR with an I.M.A.G.E clone (clone ID 4548399; Open Biosystems) as the template. Deletion mutants of TUSC4 were … how much should i weigh for my height chartWebbThe wild-type (WT), active (E40K), and dominant-negative (K179A/T308A/S473A, referred to below as AAA) human akt1 cDNAs in the pFLAG-CMV-2 vector (Sigma) or a pHM6 vector (Roche Molecular Biochemicals, Mannheim, Germany) … how much should i weigh if im 6 foot 1WebbpHM6 87228 ™ Organism Yersinia enterocolitica subsp. enterocolitica (Schleifstein and Coleman) Frederiksen Clone type Clone Applications Molecular biology Product format … how much should i weight at 5\u002710Webbexpression vector pHM6's map - (Feb/23/2005 ) i did'nt find the map information of pHM6 expression vector . who could give me information about pHM6 vector? thank you! -lwk2003- hi in J. Biol. Chem., Vol. 278, Issue 51, 51190-51202, December 19, 2003, Helen M. Reid and B. Therese Kinsella say that pHM6 came from Roche Applied Sciences. how do the sheep function as a motifWebb1 apr. 2015 · Opossum kidney (OK) cells were transfected with pHM6 empty vector (control) or a pHM6-DNPEP construct (DNPEP) and harvested 48 h later. Measurements of monomeric (G) and total cellular actin was performed by DNase I inhibition assay to determine the G/F-actin ratios, the ratio of F-actin content to total cellular protein, and … how do the sewer lines from your house workWebbpHM6 (Search Vector Database) Backbone size w/o insert (bp) 5441 Vector type Mammalian Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 … how do the seasons go in order