Daughterless-gal4
WebGal4 line directed expression of Gal4 under the control of the single intron of da in most regions of the brain, including the mushroom body (Figure 3A , A’’’ ). T wo mushroom body WebAbstract. Mammalian transcription factor 4 (TCF4) has been linked to schizophrenia and intellectual disabilities, such as Pitt-Hopkins syndrome (PTHS). Here, we show that …
Daughterless-gal4
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WebJan 3, 2012 · The daughterless-Gal4, Df(3L)vin5, rols P1027, and rols P1729 stocks were obtained from the Bloomington Drosophila Stock Center. For microarray analysis, mRNA was extracted from UAS-PAX7-FOXO1; Myosin Heavy Chain-Gal4 by the TRI zol reagent (Invitrogen) and control larvae, profiled on Affymetrix Drosophila Genome 2.0 chips, and … WebThe overarching goal of this dissertation is to import nucleus-encoded ATP6 protein into the mitochondria.This work examines the efficacies of multiple strategies in enhancing the functional outcomes of the first animal model with a stable and endogenous ATP6 mutation and shows that algal ATP6 protein provides the most promising rescue results.
WebDriving expression of UAS-dSirt6-GFP in the whole fly with daughterless-GAL4 (da-GAL4), we observed a strong nuclear GFP signal in all tissues of larvae (salivary gland shown in Fig. 1A). In addition, dSirt6-GFP protein … WebPage topic: "The F-box protein Bard (CG14317) targets the Smaug RNA-binding protein for destruction during the Drosophila maternal-to-zygotic transition". Created by: Gerald Shaw. Language: english.
WebThe GAL4/UAS system is commonly used in Drosophila to drive expression of a gene of interest (Brand & Perrimon, 1993). Tissue-specific or ubiquitous enhancers drive … WebApr 23, 2013 · Expressing CGG 90 in the retina using GMR-GAL4 drivers severely disrupted eye morphology as characterized by loss of pigmentation, neuron death (escharosis), retinal collapse, and ommatidial fusion. Expressing CGG 90 ubiquitously using Daughterless-GAL4 drivers shortened lifespan.
WebJun 25, 2002 · This approach, which exploits the UAS/GAL4 binary system to drive expression of double-stranded RNA in a defined tissue is a form of RNA interference (RNAi) that has previously been shown to block the expression of defined genes 17, 18, 19. ... daughterless-GAL4 (da-GAL4), which expresses GAL4 strongly and ubiquitously; hs …
WebFor some studies, daughterless-GAL4 was combined with UAS-Dcr2 for increased RNAi efficiency . The 4e-bp RNAi (#100739 from the Vienna Drosophila RNAi Center) is … simplicity 1657589asmWebDec 1, 2001 · As the only class I helix-loop-helix transcription factor in Drosophila, Daughterless (Da) has generally been regarded as a ubiquitously expressed binding partner for other developmentally regulated bHLH transcription factors. raymarine repairs pompano beachWebA third-chromosome daughterless-GAL4 transgene [GAL4 daG32 (25)] was used as a ubiquitous driver line. Both serpins and GAL4 daG32 transgenes were crossed into the … simplicity 1668344smWebPhosphorylation of eukaryotic translation initiation factor 2 alpha (eIF2α) by the kinase GCN2 attenuates protein synthesis during amino acid starvation in yeast, while in mammals a family of related eIF2α kinases regulate translation in response to raymarine repairs ukWebPubMed Central (PMC) simplicity 1656147WebObesity has dramatically increased in prevalence, making it essential to understand its accompanying metabolic changes. Modeling diet-induced obesity in Drosophila … simplicity 1662WebDec 12, 2024 · Construct expression was driven with daughterless -Gal-4. Full-length GFP-Step localized to the LE and tricellular junctions of the epidermis. GFP-Step∆CC+CC [N*] displayed a diffuse plasma membrane distribution with some enrichment to cell protrusions at the LE and within the epidermis (cyan arrows). simplicity 1659